Clark Gregg Agent Phil Coulson Actor Ming-Na Wen Agent Melinda May Actor Chloe Bennet Agent Daisy Johnson Actor Iain De Caestecker Agent Leo Fitz Actor Elizabeth Henstridge Agent Jemma Simmons Actor Henry Simmons Agent Alphonso "Mack" MacKenzie Actor Jason O'Mara Guest Star Gabriel Luna Guest Star Briana Venskus Guest Star Nico David Guest Star John Churchill Guest Star Adrian Quinonez Guest Star Jen Sung Guest Star Daniel Zacapa Guest Star Lilli Birdsell Guest Star Dan Donohue Guest Star Ward Roberts Guest Star Usman Ally Guest Star Deren Tadlock Guest Star Mark Daneri Guest Star Joss Whedon Executive Producer Jed Whedon Executive Producer Maurissa Tancharoen Executive Producer Jeffrey Bell Executive Producer Jeph Loeb Executive Producer Drew Z. Greenberg Writer Vincent Misiano Director