Clark Gregg Agent Phil Coulson Actor Ming-Na Wen Agent Melinda May Actor Chloe Bennet Daisy Johnson Actor Iain De Caestecker Agent Leo Fitz Actor Elizabeth Henstridge Agent Jemma Simmons Actor Henry Simmons Agent Alphonso "Mack" MacKenzie Actor John Hannah Holden Radcliffe Actor Natalia Cordova-Buckley Elena "Yo-Yo" Rodriguez Guest Star Mallory Jansen Aida Guest Star Adrian Pasdar Brigadier General Glenn Talbot Guest Star Briana Venskus Agent Piper Guest Star Maximilian Osinski Agent Davis Guest Star Zach McGowan The Superior Guest Star Ricardo Walker Agent Prince Guest Star Brennan Feonix Airman Whitley Guest Star J. Michael Evans Airman Richardson Guest Star Joss Whedon Executive Producer Jed Whedon Executive Producer, Writer Maurissa Tancharoen Executive Producer, Writer Jeffrey Bell Executive Producer Jeph Loeb Executive Producer Kevin Tancharoen Director