Buddy Ebsen Barnaby Jones Actor Lee Meriwether Betty Jones Actor John Carter Lt. John Biddle Actor Mark Shera Jedediah Romano "J.R." Jones Actor Michael Hershewe Guest Star Roger Perry Guest Star Charles Cyphers Guest Star John Kerry Guest Star Anita Minotto Guest Star Than Wyenn Guest Star Michael Masters Guest Star Peter Forster Guest Star Thom Carney Guest Star Paul Sorensen Guest Star Mann Rubin Writer Michael Caffey Director