Buddy Ebsen Barnaby Jones Actor Lee Meriwether Betty Jones Actor John Carter Lt. John Biddle Actor Mark Shera Jedediah Romano "J.R." Jones Actor Pernell Roberts Guest Star Susan Dey Guest Star Nancy Olson Guest Star Roger Perry Guest Star Frances Bergen Guest Star Richard Bakalyan Guest Star James Congdon Guest Star Alfred Avallone Guest Star Katie Hopkins Zerby Guest Star Beau Billingslea Guest Star Michael Alaimo Guest Star Larry Watson Guest Star Larry McCormick Guest Star Stanley Roberts Writer Alf Kjellin Director