Adelaide Kane Queen Mary Actor Megan Follows Queen Catherine Actor Rachel Skarsten Queen Elizabeth Actor Celina Sinden Greer Actor Craig Parker Narcisse Actor Rose Williams Princess Claude Actor Ben Geurens Gideon Blackburn Actor Dan Jeannotte James Actor Jonathan Goad John Knox Actor Spencer MacPherson King Charles Actor Will Kemp Lord Darnley Actor Adam Croasdell Lord Bothwell Guest Star Nola Augustson Lady Lennox Guest Star Richard Fleeshman Cmdr. Francis Drake Guest Star Rebecca Liddiard Princess Margot Guest Star Nick Slater Prince Henri Guest Star Ann Pirvu Nicole Guest Star Steve Byers Archduke Ferdinand Guest Star Megan Hutchings Jane Guest Star Sara Garcia Keira Guest Star Laurie McCarthy Executive Producer, Writer Frank Siracusa Executive Producer John Weber Executive Producer Brad Silberling Executive Producer Gretchen J. Berg Executive Producer Aaron Harberts Executive Producer Holly Dale Director April Blair Writer