Buddy Ebsen Barnaby Jones Actor Lee Meriwether Betty Jones Actor John Carter Lt. John Biddle Actor Mark Shera Jedediah Romano "J.R." Jones Actor Tom Mason Guest Star Tim O'Connor Guest Star Devon Ericson Guest Star Dan O'Herlihy Guest Star Richard Lynch Guest Star Ben Masters Guest Star Charles Tyner Guest Star Tara Buckman Guest Star Lyle Bettger Guest Star Victoria Young Guest Star Bob Basso Guest Star Danny Kamekona Guest Star Michael Vendrell Guest Star Kima Kahoano Guest Star Christerpher Neddels Guest Star Harry Chang Guest Star Larry Duran Guest Star Bernard Ching Guest Star Kathy Paulo Guest Star Eddie Fernandez Guest Star Ernest Chan Guest Star Elissa Dulce Hoopai Guest Star Robert L. Silva Guest Star Barton McCollough Guest Star Robert Sherman Writer Michael Caffey Director