Buddy Ebsen Barnaby Jones Actor Lee Meriwether Betty Jones Actor John Carter Lt. John Biddle Actor Mark Shera Jedediah Romano "J.R." Jones Actor Kenneth Mars Guest Star Thomas Havens Guest Star Lurene Tuttle Guest Star Mark Goddard Guest Star Lory Walsh Guest Star Julie Rogers Guest Star Madeleine Fisher Guest Star Frank Ashmore Guest Star John Steadman Guest Star Norman Jolley Writer Michael Preece Director