Clark Gregg Sarge Actor, Director Ming-Na Wen Agent Melinda May Actor Chloe Bennet Daisy Johnson Actor Elizabeth Henstridge Agent Jemma Simmons Actor Iain De Caestecker Agent Leo Fitz Actor Henry Simmons Agent Alphonso "Mack" MacKenzie Actor Natalia Cordova-Buckley Elena "Yo-Yo" Rodriguez Actor Jeff Ward Deke Shaw Actor Maximilian Osinski Guest Star Briana Venskus Guest Star Joel Stoffer Guest Star Barry Shabaka Henley Guest Star Lucas Bryant Guest Star Winston James Francis Guest Star Matt O'Leary Guest Star Brooke Williams Guest Star Glenn Keogh Guest Star Levi Meaden Guest Star Joss Whedon Executive Producer Jed Whedon Executive Producer, Writer Maurissa Tancharoen Executive Producer, Writer Jeffrey Bell Executive Producer Jeph Loeb Executive Producer