Adelaide Kane Queen Mary Actor Megan Follows Queen Catherine Actor Rachel Skarsten Queen Elizabeth Actor Celina Sinden Greer Actor Craig Parker Narcisse Actor Rose Williams Princess Claude Actor Ben Geurens Gideon Blackburn Actor Dan Jeannotte James Actor Jonathan Goad John Knox Actor Spencer MacPherson King Charles Actor Will Kemp Lord Darnley Actor Adam Croasdell Lord Bothwell Guest Star Andrew Shaver David Rizzio Guest Star Nola Augustson Lady Lennox Guest Star Paulino Nunes Cardinal Odomo Guest Star Claire Hunter Emily Knox Guest Star Laurie McCarthy Executive Producer, Writer Frank Siracusa Executive Producer John Weber Executive Producer Brad Silberling Executive Producer Gretchen J. Berg Executive Producer Aaron Harberts Executive Producer Andy Mikita Director April Blair Writer