Clark Gregg Director Phil Coulson Actor, Director Ming-Na Wen Agent Melinda May Actor Chloe Bennet Daisy Johnson Actor Iain De Caestecker Agent Leo Fitz Actor Elizabeth Henstridge Agent Jemma Simmons Actor Henry Simmons Agent Alphonso "Mack" MacKenzie Actor Natalia Cordova-Buckley Elena "Yo-Yo" Rodriguez Actor Eve Harlow Tess Guest Star Dominic Rains Kasius Guest Star Florence Faivre Sinara Guest Star Joel Stoffer Enoch Guest Star Coy Stewart Flint Guest Star Pruitt Taylor Vince Grill Guest Star Tunisha Hubbard Ava Guest Star Erika Ervin Lady Karaba Guest Star Patrick Fabian Ponarian Guest Star Myko Olivier Ben Guest Star Jay Hunter Watch Commander Guest Star Tim Sitarz Kree soldier Guest Star Isaac C. Singleton Jr. The Vicar Guest Star Samuel Roukin Faulnak Guest Star Michele Tobin Gretchen Guest Star Remington Hoffman Manston-Dar Guest Star Joss Whedon Executive Producer Jed Whedon Executive Producer Maurissa Tancharoen Executive Producer Jeffrey Bell Executive Producer Jeph Loeb Executive Producer Jim Chory Executive Producer Brent Fletcher Writer